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KMID : 0613820040140060963
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Journal of Life Science
2004 Volume.14 No. 6 p.963 ~ p.966
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The Base Sequence of ITS and Genetic Variation in Sarcodon Aspratus
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Kim Jong-Bong
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Abstract
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The sequence of ITS (partial 16S ribosomal DNA, complete ITS1, 5.8S ribosomal DNA and ITS2, and partial 28S ribosomal DNA) was analysed by PCR and autosequencing in Sarcodon aspratus. The ITS lenght of S. aspratus was 716 base pair. As this sequence compared with other reports on S. aspratus (acc No AF335110), the sequence variation based on nucleotid deletion and substitution was 1.8%. This nucleotide variation rate in same species was very higher than in other species. Also, the sequence varitation rates between this S. aspratus and S. imbricatus, and S. squamus were 8% and 10%, respectivly. This results suggested that the high sequence variation of S. aspratus might be caused specific host and inhabitat enviroment which limited gene flow
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KEYWORD
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Sarcodon aspratus, ITS sequence, Genetic Variation
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